Effect of Explant Type, Benzyl amino purine (BAP), Type of Media and Light Intensity on In Vitro Shoot Multiplication of Chrysanthemum morifolium Ramat

  • Yousra Faisal Elshaikh Babiker College of Agricultural Studies, Sudan University of Science and Technology, Shambat, Sudan
  • Seifeldin Ali Mohamed Department of Horticulture, Faculty of Agriculture, University of Khartoum, Shambat, Sudan
  • Tagelsir Ibrahim Mohamed Idris College of Agricultural Studies, Sudan University of Science and Technology, Shambat, Sudan

Abstract

This study was conducted to evaluate the effect of explant type (shoot tips and lateral buds); different concentrations of the cytokinin 6-Benzyl amino purine (BAP) at 1, 2, 4, 6, 8 mg/l; the organic acid (ascorbic acid) at 20 and 40 mg/l; the amino acid L-glycine at 30 and 60 mg/l; liquid medium overlay onto solid media (0, 4, 8, 12, 16 ml of liquid medium)  and light intensity (0, 1.4, 7, 14 µmol m-2 s-1) on in vitro shoot multiplication in Chrysanthemum morifolium. The completely randomized design was used in these experiments. The multiplication parameters collected were percentage of explants with shoots, number of shoots/explant, shoot length, number of leaves/explant and number of nodes/shoot. Data were subjected to analysis of variance. Means were separated by Duncan’s multiple range test, and T-test.  With regard to the parameter percentage of explants with shoots, all levels of all factors tested resulted in 100% explants with shoots. For the other parameters there was no significant difference between shoot tips and lateral buds. Significant differences were recorded as a result of BAP concentrations. The low concentrations of BAP (1 and 2 mg/l)  significantly stimulated multiplication parameters. The supplement of ascorbic acid at low concentration (20 mg/l) significantly enhanced multiplication. Incorporation of L-glycine at the concentration of 30 or 60 mg/l in the medium enhanced multiplication significantly compared to the control. Addition of liquid medium onto the surface of solid medium  stimulated all growth parameters significantly. Cultures incubated under dark condition resulted in significant increase in multiplication but the resultant shoots exhibited clear symptoms of etiolation. The study concluded that the use of lateral buds as explants, BAP at 1 mg/l, ascorbic acid at 20 mg/l, glycine at 30 mg/l, 16 mls of  liquid medium  as overlays onto solid medium and light intensity of 14  µmol m-2 s-1  is suitable within a protocol for micropropagation of Chrysanthemum morifolium.  

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Published
2022-09-29
How to Cite
ELSHAIKH BABIKER, Yousra Faisal; ALI MOHAMED, Seifeldin; MOHAMED IDRIS, Tagelsir Ibrahim. Effect of Explant Type, Benzyl amino purine (BAP), Type of Media and Light Intensity on In Vitro Shoot Multiplication of Chrysanthemum morifolium Ramat. Gezira Journal of Agricultural Science, [S.l.], v. 19, n. 1, p. 31-41, sep. 2022. ISSN 1728-9556. Available at: <http://journals.uofg.edu.sd/index.php/gjas/article/view/1914>. Date accessed: 04 dec. 2022.
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Articles