Isolation and identification of Bacillus thuringiensis from Sudan soils

Abdulwahab A. . Ahmed, Hamid A .Dirar

Abstract


Bacillus thuringiensis is a gram positive, aerobic and spore-forming bacteria. It produces pro-toxins that show insecticidal property against larvae of different orders including Lepidoptera, Diptera and Coleoptera (Carlberg, 1986). The earliest discovery of a spore-forming bacterium with insecticidal mechanism  was from a diseased silkworm, Bombyx mori by a Japanese bacteriologist named Ishwata in the year 1901. Later, in the year 1911, a similar organism was reported from Mediterranean flour moth (Anagasta kachinella) in Germany and it was given the name B. thuringiensis Berliner (Baum et al., 1999). Subsequently, thousands of strains were reported to exist worldwide (Lereclus et. al., 1993).

It is generally accepted that the primary habitat of the majority of Bacillus species is the soil where they were considered part of zymogenous bacterial flora. The species B. thuringiensis is commonly found in the soil world-wide (Lee et al., 2001). This study was initiated to screen for strains of Bacillus thuringiensis from soils of the Sudan at different sites. The experiment was conducted in the Microbiology laboratory at the Faculty of Agriculture, University of Khartoum in the year 2003.


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