Establishment of an encapsulation technique for in vitro propagated plantlets of banana (Musa AAA) cv. Albeely

Mohammed H. A. Othman, Mohamed A. Ali, Igbal A. Abdellatif

Abstract


Edible triploid banana are vegetatively propagated by suckers because viable seeds are not usually produced in these cultivars. New and effective means of propagating bananas would be advantageous, over the conventional use of sucker material, for germplasm maintenance, exchange and transportation. In vitro culture of floral apices or vegetative meristem is the most promising propagation method available to date (Ganapathi et al., 1992). Synthetic seeds are defined as artificially encapsulated somatic embryoes, shoot buds, cell aggregates, or any other tissue that can be used for sowing as a seed, or other in vitro-derived tissue capable of conversion into plantlets under in vitro or in vivo conditions and that retain this potential after storage (Ganapathi et al., 1992). Nevertheless, to reach such objective, the encapsulation technique must be firstly improved regarding its composition and conditions for short term storage. In the case of banana (Musa AAA), the use of synthetic seed technique may improve the quality of the plantlets and reduce the production costs. This technique was successfully employed in the cvs. ‘Basrai’ by Ganapathi et al. (1992).

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