Effects of cytokinins and auxins on the micropropagation of a local grapevine (Vitis vinifera L.) cultivar, using nodal explants

Osman A. Ali, Mohamed A. Ali, Abdel Moneim M. Salama


This study was conducted in the Plant Tissue Culture Unit of the Agricultural Research Corporation, Wad Medani, Sudan, during the years 2006- 2007. The objectives were to develop an in vitro technique for the propagation of a local cultivar of grapevine. The basal Murashige and Skoog medium was supplemented with benzylaminopurine (BAP) and kinetin both at concentrations of 0.0, 0.25, 0.5, 1.0, 2.0, 4.0, 6.0, and 8.0 mg/l. Isopentenyladenine (2iP) and BAP were also used at concentrations of 0.0, 1.0, 1.5, and 2.0 mg/l.  The interaction effects of indole acetic acid (IAA) at concentrations of 0.0, 0.25, 0.5 and 1.0 mg/l with BAP at 1.0 or 2.0 mg/l and indole butyric acid (IBA) at concentrations of 0.0, 0.1, 0.3, and 0.5 mg/l with 2iP at 1.0 or 2.0 mg/l were also tested. For in vitro rooting, plantlets were cultured on MS medium supplemented with different concentrations of NAA (0.0, 0.25, 0.5, and 1.0 mg/l). The best morphogenesis of shoots was achieved on MS medium supplemented with BAP at 1.0-1.5 mg/l. The morphogenetic response increased with time and the best response was attained after six weeks. Higher concentrations of BAP (2.0-8.0 mg/l) resulted in abnormal growth of plantlets. The combination of BAP with IAA did not improve shoot morphogenesis. The morphogenesis of the nodal explants was similar on kinetin and 2iP which resulted in a single shoot per explant. The combinations of 2iP at different concentrations with IBA did not improve shoot morphogenesis compared to 2iP alone. The best rooting of regenerated shoots of grapevine was achieved on MS medium supplemented with 0.25 and 0.5 mg/l NAA after four weeks.

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