Micrografting technique for cloning Hashab [Acacia senegal (L.) Willd]: Effect of rootstock age, scion length, scion source, sucrose and light duration

Ghada S. Altayeb, Mohamed A. Ali


Hashab [Acacia senegal (L.) Willd] is considered as an important tree species in Sudan which produces gum Arabic. It is mainly propagated by seeds, which result in variation in yield due to segregation. This study was established to develop an in vitro micrografting technique for cloning Hashab trees. Five experiments were conducted during 2012 to investigate the effects of age of rootstock seedling, scion length, scion source, sucrose, and light duration on the success of micrografting of Hashab in a completely randomized design with ten replicates. Two and four weeks- old seedlings were tested as rootstocks. They were grafted with scions obtained from the greenhouse. The two weeks-old seedlings had significantly higher percentage of successful grafts, and scion morphogenesis. Different scion lengths (1-1.5 cm and 2.5-3cm) were tested.  Scions 1-1.5cm long had significantly higher rates of successful micrografts. Scions obtained from different sources (greenhouse, in vitro plantlet, and mature tree) were evaluated and those obtained from the greenhouse gave significantly higher percentages of successful grafts, number of leaves, number of nodes, and an increase in shoot length. Different concentrations of sucrose (20, 30, 40, 50, and 60 g/l) were tested and grafted plants at 40g/l sucrose gave significantly higher percentages of successful grafts. Incubation of micrografted seedlings under continuous light for two weeks gave significantly higher percentages of successful grafts.


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