Effect of 6-benzylaminopurine, naphthaleneacetic acid and activated charcoal on rose micropropagation using nodal explants

Hala M. Abdallah, Mohamed A. Ali


Rose is one of the most important commercial flower crops. It is used in the floriculture and cut flower industry, perfume, cosmetic and medicinal purposes in many regions of the world. Conventional methods of rose propagation are slow with a low percentage of success. The objective of this study was to develop a protocol for in vitro propagation of rose, cv. Sara, using nodal explants. Three experiments were conducted during 2012.  In the first experiment, the effects of different concentrations of  6-benzylaminopurine (BAP) (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) were tested on nodal explants of rose, cv. Sara. Different concentrations of naphthaleneacetic acid (NAA) (0.0, 0.1, 0.2 and 0.4 mg/l) combined with 1.0 mg/l BAP were tested in the second experiment. Factorial combinations between BAP at 0.0, 1.0 and 2.0 mg//l and activated charcoal (AC) (0.0 and 3.0 g/l) were tested. Completely randomized design was used in this study with five replicates and four explants per replicate. BAP at 1.0 mg/l was the best concentration for in vitro morphogenesis of rose, cv. Sara. The results showed that combinations of NAA with 1.0 mg/l BAP significantly reduced the number of shoots per explant. Addition of AC to MS medium devoid from BAP increased the number of shoots. BAP at 1.0 mg/l without AC was the best for shoot multiplication. AC with 2.0 mg/l BAP resulted in the maximum number of roots per plantlet.

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