Association of Vitamin D Receptor Gene Polymorphism (Taq1) in Sudanese Children with Type 1 Diabetes Mellitus at Gezira State, Sudan.

  • Mohammed A Hamza Department of Biochemistry and nutrition, Faculty of Medicine, University of Gezira
  • Khalid E. Khalid Department of Biochemistry and nutrition, Faculty of Medicine, University of Gezira
  • Osman K Saeed Department of Medicine, Faculty of Medicine, University of Gezira.
  • Huda M Haroun Department of Pediatric, Faculty of Medicine, University of Gezira


Introduction: Diabetes mellitus describes a metabolic disorder of multiple aetiology characterized by chronic hyperglycemia with disturbances of carbohydrate, fat and protein metabolism resulting from defects in insulin secretion, insulin action, or both.

Objectives: This study aimed to determine the frequency of selected VDR gene polymorphismTaq1(rs731236) single nucleotide polymorphism in diabetic and non-diabetic children to assess its contribution to the susceptibility to type 1 diabetes (T1D).

Methods: One hundred children with T1D were enrolled in this study; 50 healthy non-diabetic children served as a control group for detection of VDR gene polymorphism Taq1. This polymorphism was detected by using the polymerase chain reaction (PCR) and restriction fragment polymorphism (RFLP) analysis.

Results: There was a significant difference between frequencies of Taq1(t allele) ((P= 0.05; Odd Ratio = 0.412; 95% CI 0.182 - 0.932).

Conclusion: VDR TaqI polymorphism was associated with T1D in Sudanese children.

يوصف مرض السكري بأنه من المسببات المرضية المتعددة التي تتميز بفرط  سكر الدم المزمن واضطراب في التمثيل الغذائي ( اضطرابات من الكربوهيدرات والدهون واستقلاب البروتين) الناتج عن نقص في إفراز الأنسولين، عمل الانسولين أو كليهما.

هدفت هذه الدراسة لتحديد تكرارات تعدد شكل الجين المستقبل لفايتمين د في مجموعتي المرضي والأصحاء ولتقييم دوره في الإصابة بالسكري عند الأطفال. اشتملت هذه الدراسة علي 100طفل مصابين مرض السكري و50 أصحاء من نفس العمر.تضمنت هذه الدراسة تكرارات تعدد شكل الجين المستقبل لفايتمين د(Taq1)  في مجموعتي المرضي والأصحاء . أظهرت هذه الدراسة ان تكرار الاليل (t) في الجين المسقبل لفايتمين د  يختلف اختلاف ذو معني  عند مجموعة الأصحاء مقارنة  بالمرضي مما يعني ان لهما دور في الحماية من الاصابة بالسكري عند الأطفال .

خلصت هذه الدراسة الي ان التعدد في الشكل الجيني لمسقبل فايتمين د (Taq1) لهما علاقة بالإصابة بالسكري النوع الأول عند الاطفال السودانيين.


1. Pozzilli, P. and Di Mario, U. (2001). Autoimmune diabetes not requiring insulin at diagnosis (latent autoimmune diabetes of the adult): definition, characterization, and potential prevention. Diabetes Care, 24: 1460–1467.
2. Steck,AK. and Rewers, MJ. (2011) Genetics of type 1 diabetes. Clin Chem57: 176-185.
3. Valdivielso, JM.and Fernandez, E. (2006) Vitamin D receptor polymorphisms and diseases Clin Chim Acta,371: 1-12.
4. Kulie, T. Groff, A. Redmer, J. Hounshell, J. and Schrager, S. (2009) Vitamin D: an evidence-based review. J Am Board Fam Med,22: 698-706.
5. Kato, S. (2000) The function of vitamin D receptor in vitamin D action. J Biochem,127: 717–722.
6. Turpeinen,H. Hermann, R. Vaara, S. Laine ,AP. and Simell, O. (2003) Vitamin D receptor polymorphisms: no association with type 1 diabetes in the Finnish population. Eur J Endocrinol,149: 591-596.
7. Guo, SW. (2006) Meta-analysis of vitamin D receptor polymorphisms and type 1 diabetes: A huge review of genetic association studies. Am J Epidemiol,164: 711-724.
8.;g=EN SG00000111424;r=12:133275309-133275309;v=rs731236;vdb=variation; vf=515885.
9. Tokita, A., Matsumoto, H., Morrison, N.A., Tawa, T., Miura, Y., Fukamachi, K., Mitsuhashi, N., Irimoto, M., Yamamori, S., Miura, M., Watanabe, T., Kuwabara, Y., Yabuta, K. and Eisman, J. A. (1996). Vitamin D receptor alleles, bone mineral density and turnover in premenopausal Japanese women. J Bone Miner Res, 11: 1003-1009.
10. Ozfirat, Z. and Chowdhury, T. A. (2010). Vitamin D deficiency and type 2 diabetes. Postgrad Med J, 86:18–25.
11. Mohammadnejad, Z., Ghanbari, M., Ganjali, R., Afshari, J.T., Heydarpour, M., Taghavi, S. M., Fatemi, S., Rafatpanah, H.(2012). Association between vitamin D receptor gene polymorphisms and type 1 diabetes mellitus in Iranian population. Mol Biol Rep, 39 (2):831-837.
12. Fassbender, W. J., Goertz, B., Weismuller, K., Steinhauer, B., Stracke, H., Auch, D., Linn, T. and Bretzel, R. G.(2002). "VDR gene polymorphisms are overrepresented in german patients with type 1 diabetes compared to healthy controls without effect on biochemical parameters of bone metabolism." Horm Metab Res, 34(6): 330-337.
13. Taverna, M. J., Sola, A., Guyot-Argenton, C., Pacher, N., Bruzzo, F., Slama, G., Reach, G. and Selam, J. L.(2002). Taq I polymorphism of the vitamin D receptor and risk of severe diabetic retinopathy. Diabetologia, 45(3): 436-442.
14. Kamel, MM. Fouad, SA. Salaheldin, O.and Aeraa, El. (2014) Impact of vitamin D receptor gene polymorphisms in pathogenesis of Type-1 diabetes mellitus. Int J Clin Exp Med7: 5505-5510
15. Rizk, MM. Zakaria, NH.And Elshazely, WG (2014) Study of vitamin D receptor (VDR) gene polymorphisms among Egyptian cohort patients with different stages of colorectal cancer. J Cancer Ther5: 253-263.
16. Abu el Maaty, MA. Hassanein, SI. Sleem, HM. and Gad, MZ. (2015) Vitamin D receptor gene polymorphisms (TaqI and ApaI) in relation to 25-hydroxyvitamin D levels and coronary artery disease incidence. J. Recept. Signal Transduct35: 391-395.
17. Bonakdaran, S. Abbaszadegan, MR. Dadkhah, E. and Khajeh-Dalouie, M. (2012) Vitamin D receptor gene polymorphisms in type 1 diabetes mellitus: a new pattern from Khorasan province, Islamic Republic of Iran/Polymorphismes du gène du récepteur de la vitamine D et diabète de type 1: un nouveau modèle dans la province de Khorasan (République islamique d’Iran). East Mediterr Health J18: 614.
18. Haddad, S. (2014) Vitamin-D receptor (VDR) gene polymorphisms (Taq-I & Apa-I) in Syrian healthy population. Meta Gene2: 646-650.
19. Bianco, MG. Minicucci, L. Calevo, MG.and Lorini, R. (2004) Vitamin D receptor polymorphisms: are they really associated with type 1 diabetes.Eur. J Endocrinol151: 641.
How to Cite
A HAMZA, Mohammed et al. Association of Vitamin D Receptor Gene Polymorphism (Taq1) in Sudanese Children with Type 1 Diabetes Mellitus at Gezira State, Sudan.. Gezira Journal of Health Sciences, [S.l.], v. 14, n. 2, dec. 2018. ISSN 1810-5386. Available at: <>. Date accessed: 07 apr. 2020.