Seroprevalence of Brucellosis Associated with Abortion among Sudanese Pregnant Women attending Omdurman Maternity Hospital, Sudan
Background: Human Brucellosis remains the most common zoonotic disease endemic in the Mediterranean area, Middle East, Southwest Asia, and parts of Latin America. The disease is endemic in the Sudan and was reported as early as 1908. It is a commonly misdiagnosed febrile disease with malaria and typhoid fever. Brucellosis is a multi-systemic disease characterized with fever, osteoarticular involvement, neuropathies, chorea, and meningoencephalitis, gastrointestinal, hepatobiliary, genitourinary, musculoskeletal, cardiovascular, pleural effusion and pneumonia. Due to increase rates of spontaneous abortion, premature delivery and intrauterine infection with fetal death caused by this disease, there is need for urgent intervention to reduce maternal mortality rate and increase child survival. This study was designed to investigate the seroprevalence of Brucellosis associated abortion among Sudanese.
Objective of the Study: To investigate the seroprevalence of Brucellosis associated abortion among Sudanese attending Omdurman Maternity Hospital, Sudan.
Materials and Methods: A cross-sectional study was carried out among 120 pregnant women who have aborted and 21 pregnant women who have not aborted who attended Omdurman Maternity Hospital during the period from August to October (2015). Serum samples were collected to detect the presence of the Brucella anti bodies (BAbs) using Rose Bengal plate Test (RBPT) as screening test and Serum Agglutination Test (SAT) as confirmatory test. A structured questionnaires was designed to record demographical clinical data.
Results: Prevalence of Brucella disease was found to be 1.7%(2/120) in pregnant who have aborted and increased significantly with milk consumption, cheese eating, and yoghurt consumption.
Conclusion: These findings demonstrated the prevalence of Brucellosis associated abortion among Sudanese women, so that the coordination between medics and researchers started in this field.
2. 2-Baud, D.; Peter, O.; Langel, C.; Regan, L.; Greub, G..Seroprevalence of Coxiellaburnetii and Brucella abortus among pregnant women. ClinMicrobiol Infect; (2009).15: 499–501.
3. 3-Haseeb, M. A.. Undulant fever in the Sudan . Sud. J. Trop. Med., .(1950).53:241.
4. 4-Simpson, R. J. S. Malta fever from the Blue Nile . J.Roy.ArmyMed Corps., .(1908).11:593.
5. 5-Bennett, S. G. J.. Annual Report of the Sudan Veterinary Service .(1943)PP.29-32.
6. 6-Dafalla, E. N.. Incidence of animal and human brucellosis in the Sudan . Sudan .J Vet. Sci. and Anim. Husb.,(1962)3:80-89 .
7. 7-Brooks, GF.; Butel, J.S.; Morse, S.A. The Brucellae, Jawetz, Medical Microbiology, twenty second edition, McGraw Hill,(2001). 246-248.
8. 8-Joklik, Willett.; Amos, Wilfert.; Wolfgang, K.. Brucella, Zinsser Microbiology, 20th edition, McGraw Hill,pp(1992) 609-661.
9. 9-Muflihanah, H.; Hatta, M.; Rood, E.; Scheelbeek, P.; Abdoel, T.H.; Smits, H.L.. “Brucellosis seroprevalence in Bali cattle with reproductive failure in South Sulawesi and Brucella abortusbiovar 1 genotypes in the Eastern Indonesian archipelago,” BMC Veterinary Research,.(2013).vol. 9, article 233.
10. 10-Boukary, A.R.; Saegerman, C.; Abatih, E. (2013). “Seroprevalence and potential risk factors for Brucella spp. infection in traditional cattle, sheep and goats reared in urban, periurban and rural areas of Niger,” PLoS ONE, 8(12):83175.
11. 11-Dean, A.S, Crump, L.; Greter, H.. Global burden of human brucellosis: a systematic review of disease frequency. PLoSNegl Trop Dis;6(10):1865.
12. 12-O'Callaghan, D. (2013).“Novel replication profiles of Brucella in human trophoblasts give insights into the pathogenesis of infectious abortion,” The Journal of Infectious Diseases,(2012).207(7): 1034–1036.
13. 13-Sperry, J.F.; Robertson, D.C.. “Erythritol catabolism by Brucella abortus,” Journal of Bacteriology,(1975).121(2): 619–630.
14. 14-Salcedo, S.P.; Chevrier, N.; Lacerda, T.L.S.. “Pathogenic brucellae replicate in human trophoblasts,” Journal of Infectious Diseases,(2013)207(7): 1075–1083.
15. 15-Pappas, G.; Akritidis, N.; Bosilkovski, M.; Tsianos, E. Brucellosis. N Engl J Med;(2005).352:2325-2336.
16. 16-Ogredici, O.; Erb, S.; Langer, I.. Brucellosis reactivation after 28 years. Emerg Infect Dis; (2010)16: 2021–2022.
17. 17-Kurdoglu, M.; Adali, E.; Kurdoglu, Z.. Brucellosis in pregnancy: a 6-year clinical analysis. Arch GynecolObstet(2010); 281: 201–206.
18. 18-Diaz, R.; Casanova, A.; Ariza, J.; Moriyon, I.. The rose bengal test in human brucellosis: a neglected test for the diagnosis of a neglected disease. PLoSNegl Trop Dis;(2011)5: 950.
19. 19-Taleski, V.. An Overview of Introducing Various Laboratory Tests for Diagnosis of Human Brucellosis in the Republic of Macedonia. Macedonian Journal of Medical Sciences. Sep(2010) 15; 3(3):239-245.
20. 20-El Sherbini, A.; Kabbash, I.; Bassili, A.; El Shennawy, S.; El Assal, MD. Risk factor and diagnostic criteria of Brucellosis in an endemic area in Egypt. Infectious Diseases in Clinical Practice.; (2005).13(6): 295-299.
21. 21-Morgan, W.J.B.. The serological diagnosis of bovine brucellosis, Veterinary Record,(1967)80: 612-620.
22. 22-Abo-Shehada, M.N. and Abu-Halaweh, M. Seroprevalence of brucellosis among women with miscarriage in Jordan. Eastern MediterraneanHealth J.(2011).17(11):871-874.
23. 23-Nassajı., Mohammad; Rahbar, Nahid; Ghorbanı, Raheb; Lavaf, Sabahat. The Role of Brucella Infection Among Women with Spontaneous Abortion in an Endemic Region. Journal of the Turkish-German Gynecological Association, .(2008).9(1):20-23.
24. 24-Nadine Rujeni and LéonidasMbanzamihigo. Prevalence of Brucellosis among Women Presenting with Abortion/Stillbirth in Huye, Rwanda.Journal of Tropical Medicine, 2014 (740479):http://dx.doi.org/10.1155/2014/740479
25. 25-Kassiri, H.; Amani, H.; and Lotfi, M. “Epidemiological, laboratory, diagnostic and public health aspects of human brucellosis in western Iran,” Asian Pacific Journal of Tropical Biomedicine,(2013). 3: pp 589–594.
26. 26-Chernysheva, MI.; Gubina, E.A.; Zheludkov, M.M.; Perekopskaia, T.I.Isopol'zovaniekislogo antigen roz-bengal v plastinchatoireakstiiaggliutinatsiipribrutsellezeuliudei. ZhMikrobiolEpidemiol Immunobio;(1980).l:84–88.
27. 27-Konstantinidis, A.; Minas, A.; Pournaras, S.; Kansouzidou, A.; Papastergiou, P. . Evaluation and comparison of fluorescence polarization assay with three of the currently used serological tests in diagnosis of human brucellosis. Eur J ClinMicrobiol Infect Dis;(2007)26:715–721.