Direct Extraction and Assessment of Genomic DNA of Mycetoma Fungi from Black-grains Specimen

  • Elhadi Abdalla Ahmed Faculty of Medical Laboratory Sciences, University of Gezira
  • Bakri Y M Nour BNNICD, University of Gezira, Sudan
  • Adam D Abakar Department of Medical Parasitology, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan
  • Gamal M. A. Ahmed Faculty of Medicine, University of Gezira, Sudan
  • Ahmed E Ahmed Ministry of Health, Gezira State, Sudan


Background: Direct isolation of genomic DNA of mycetoma fungi from black-grains achieve rapid diagnosis and may overcome culture disadvantages. Objectives: This study aimed to isolate and assess the DNA of mycetoma fungi using black-grains and to apply amplification of ITS region and nucleotide sequences. Methods: CTAB method was followed by manual homogenization alternatively to liquid nitrogen and glass beads disruption to obtain the genomic DNA. Results: Yielded DNA concentrations vary from 1.50 to 47.97 μg/ml (mean 10.09 μg/ml) while the optimum DNA purity recorded with 75.8% of specimens (n=69/91).Successful amplification of ITS region was done using pan-fungal primers (ITS4/5) with 90.1 (n=82/91)percentage. Species nucleotide sequences were detected with 67 (94.4%) amplicons from a total of 71.Conclusion: The study recommended using of black-grain specimens for DNA extraction of mycetoma fungi parallel with culture to insure rabid diagnosis and identification.


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How to Cite
AHMED, Elhadi Abdalla et al. Direct Extraction and Assessment of Genomic DNA of Mycetoma Fungi from Black-grains Specimen. Gezira Journal of Health Sciences, [S.l.], v. 17, n. 1, p. 24-36, feb. 2022. ISSN 1810-5386. Available at: <>. Date accessed: 04 dec. 2022.