THE INFLUENCE OF THE STORAGE TEMPERATURE ON THE ACTIVITY OF THE RECONSTITUTED ORAL AMPICILLIN SUSPENSIONS

  • Mirghani Abd Elrahman Yousif Faculty of Pharmacy – University of Gezira - Sudan
  • Asim Farouk Faculty of Pharmacy – University of Gezira - Sudan

Abstract

The following sets of experiments were conducted to investigate the extent of degradation of ampicillin antibiotic in suspension formulations stored at two different temperatures. The only variable in these experiments was the storage temperature. Oral ampicillin powders were reconstituted with distilled water and stored at two different temperatures of 5o and 35oC respectively representing the refrigeration and average room temperature in Sudan. The reconstituted ampicillin suspensions were assayed for initial drug contents, and then daily for a period of seven days in order to determine the effect of the storage temperature on the stability of reconstituted oral ampicillin suspensions. The drug contents in the different samples were determined using the microbiological cup plate method and the chemical spectrophotometric technique. The results obtained showed a remarkable decrease in the activity of the oral ampicillin suspensions stored at room temperature, when compared with the refrigerated ones. Recommendation of a unit-dose sachet package of ampicillin oral powder to be reconstituted at the time of intake was suggested.

References

1. Allen LV, Stiles ML, Prince SJ, and Smeeding J, Stability of 14 drugs in the latex reservoir of an elastomeric infusion device. Am J Health Syst Pharm., 1996; 53 (Nov.15); 40-43.
2. Allwood MC and Brown PW, Stability of ampicillin infusions in unbuffered and buffered saline. Int J Pharm; 1993; 97: 219-222.
3. Das Gupta V and Shah KA, Stability of ampicillin sodium and penicillin G
potassium solutions using HPLC. Can J Pharm Sci; 1981; 16(1): 61-65.
4. Stjernstrom G, Olson OT, Nygvist H and Lundgren P, Studies on the stability and compatibility of drugs in infusion fluids. Acta Pharm Suec; 1978; 15: 30-50
5. Juste JL. et al., Ampicillin solution stability for outpatient antibiotic therapy (OPAT) in patients with Enterococcal endocarditis. Farm Hosp 1999; 23 Esp Congr:21
6. Brown LH, Krumperman K and Fullagar CJ, Out-of-hospital medication storage temperature: a review of the literature and directions for the future. Prehosp Emerg Care 2004 Apr-May; 8(2): 200-6.
7. Allegra JR; BrennanJ; Lanier V; Lavery R and Mackenzie B. Storage temperatures of out-of-hospital medications. Acad Emerg Med., 1999 Nov;6(11):1098-103.
8. Smith JWG; de Grey G; Patal VJ.
Analyst 92, 247.
9. Stella VJ, Chemical and physical basis determining the instability and incompatibility of formulated injectable drugs. J Parentr SciTechnol 1986; 40: 142-63.
10. Chapi-Robertson K and Edberg S. Measurement of antibiotics in human body fluids: techniques and significance. In: Lorian V, ed. Antibiotics in laboratory medicine. 3rd edition. Baltimore: Wilkins and Wilkins; 1991: 295-366.
Verdon E, Fuselier R, Hurtaud-PesselD, Couedor P, Cadieu N and Laurentie M, Stability of antibiotics residues in meat during storage ampicillin. J Chromatogr A 2000 Jun 16; 882(1-2): 135-43.
Published
2005-01-01
How to Cite
YOUSIF, Mirghani Abd Elrahman; FAROUK, Asim. THE INFLUENCE OF THE STORAGE TEMPERATURE ON THE ACTIVITY OF THE RECONSTITUTED ORAL AMPICILLIN SUSPENSIONS. Gezira Journal of Health Sciences, [S.l.], v. 1, n. 2, jan. 2005. ISSN 1810-5386. Available at: <http://journals.uofg.edu.sd/index.php/gjhs/article/view/171>. Date accessed: 17 feb. 2019.
Section
Articles