• Shehab H. Mehenna Al-Dulaymi Faculty of Medicine , University of Gezira , Sudan
  • Hani T. Azzawi Faculty of Medicine , University of Muta, Jordon


Objective: To investigate the metabolic activity of the adult rabbit choroid plexus, using succinate dehydrogenase, phosphorylase and α-naphthylacetate esterase as histochemical markers of, the aerobic, glycolytic, and lipolytic pathways,  respectively.

Methods: Coverslip-mounted choroid plexus sections of adult rabbits were stained histochemically for the above enzymes. To characterize the esterase isoform(s), sections were incubated with various esterase modifiers before identification of the esterase activity. Sections of liver and kidney (controls) were simultaneously treated as for choroid plexus sections.  Results: Strong reactivity of the choroidal epithelium for both succinate dehydrogenase and esterase was readily detectable, while phosphorylase activity was virtually absent. In contrast to the B-isoform of esterase characteristically dominated the controls, the choroidal esterase activity was attributed mainly to C-isoform.                                                                                                                                                                    Conclusion: The results suggest that the energy required for CSF formation by the adult choroid plexus is derived almost exclusively from aerobic oxidation, including fat metabolism. The high esterase activity in the choroid plexus , and in particular the unique pattern of the choroidal esterase versus the esterase of the controls, were interpreted to offer a potential target for future inhibitors of the energy of fat metabolism and thereby for CSF reduction.


1. Keep RF, Xiang J, Ulanski LJ, Brosius FC, Betz AL. Choroid plexus ion transporter expression and cerebrospinal fluid secretion. Acta Neurochir Suppl Wien 1997; 70: 279-281.
2. Speake T, Whitwell C, Kajita H, Majid A, Brown PD. Mechanisms of CSF secretion by the choroid plexus. Microsc Res Tech 2001; 52: 49-59.
3. Wang D, Kaur C. Choroid plexus epithelial cells in adult rats show structural alteration but not apoptosis following an exposure to hypobaric hypoxia. Neurosci Lett 2001; 297: 77-80.
4. Cornford EM, Varesi JB, Hyman S, Damian RT, Raleigh MJ. Mitochondrial content of choroid plexus epithelium. Exp Brain Res 1997; 116: 399-405.
5. Dziegielewska KM, EK J, Habgood MD, Saunders NR. Development of the choroid plexus. Microsc Res Tech 2001; 52: 5-20.
6. Tennyson VM, Pappas GD. The fine structure of the choroid plexus: Adult and developmental stages. In: Progress in Brain Research. Brain Barrier Systems: Lajtha A, Ford DH (Eds.).Amsterdam: Elsevier, 1967; 29: 63-85.
7. Shuangshoti S, Netsky MG. Human choroid plexus: Morphologic and histochemical alterations with age. Am J Anat 1970; 128: 73-96.
8. Segal MB. Transport of nutrients across the choroid plexus. Microsc Res 2001; 52: 38-48.
9. Lindvall-Axelsson M, Owman C. Actions of sex steroids and corticosteroids on rabbit choroid plexus as shown in transport capacity and rate of cerebrospinal fluid formation Neurol Res 1990; 12: 181-186.
10. Fisone G, Synder GL, Fryckstedt J, Caplan MJ, Aperia A, Greengard P. Na+, K+ - ATPase in the choroid plexus. Regulation by serotonin/protein kinase C pathway. J. Biol Chem 1995; 270: 2427-2430.
11. Kim CS, Hoppel CL. Carnitine palmitoyltransferase activity in the rabbit choroid plexus: its possible function in fatty acid metabolism and transport. Neurosci Lett 1992; 140: 13-15.
12. Bourre JM, Dinh L, Boithias C, Dumont O, Piciotti M, Cunnane S. Possible role of the choroid plexus in the supply of brain tissue with polyunsaturated fatty acids. Neurosci Lett 1997; 224: 1-4.
13. Kvitnitskaia RT, Shkapenko AL. Acomparative ultracytochemical and biochemical study of the ATPases of the choroid plexus in aging. Tsitologiia 1992; 34: 81-87.
14. Parkkila S, Parkkila AK, Rajaniemi H, Shah GN, Grubb JH, Waheed A, Sly WS. Expression of membrane-associated carbonic anhydrase XIV on neurons and axons in mouse and human brain. Proc Natl Acad Sci USA .2001; 98: 1918-1923.
15. Catala M. Carbonic anhydrase activity during development of the choroid plexus in the human fetus. Childs Nerv Syst 1997; 13: 364-368.
16. Egertova M, Cravatt BF, Elphick MR. Fatty acid amide hydrolase expression in rat choroid plexus: possible role in regulation of the sleep- inducing action of oleamide. Neurosci Lett 2000; 282: 13-16.
17. Eakin TJ, Antonelli MC, Malchiodi EL, Baskin DG, Stahl WL. Localization of the plasma membrane Ca(2+) ATPase isoform PMCA3 in rat cerebellum, choroid plexus and hippocampus. Brain Res Mol Brain Res 1995; 29: 71-80.
18. Schachenmayr W. Uber die Ertwicklungvan Ependym und Plexus chorioideus der Ratte. Z. Zellforsch 1967; 77: 25-63.
19. Kim CS, Roe CR, Ambrose WW. L-Carnitine prevents mitochondiral damage induced by octanoic acid in the rat choroid plexus. Brain Res 1990; 536: 335-338.
20. Al-Dulaymi HS. A modification in Hansson’s technique for histochemical demonstration of carbonic anhydrase activity in tissue sections. The Yemeni J Med Sciences 2001; 1: 84-89.
21. Ibrahim MZM, Castellani P. Demonstration of phosphorylase in the rat brain. Histochemie 1968; 16: 9-14.
22. Roskoski R. Biochemistry, Ist ed, Philadelphia, WB Saunders Company 1996, pp. 150 & 500.
23. Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper’s Biochemistry, 25th ed, A Lange Medical book. Appleton & Lange, California 2000; pp. 137, 199 & 763.
24. Van-Lith HA, Meijer GW, Van-Zutphen LFM, Beynen AC. Plasma esterase-1 (ES-1) activity is increased in rats fed high-fat diets. Lipids 1989; 24: 86-88.
25. Van-Lith HA, Meijer GW, Van-der Wouw MJA, Den-Bieman M, Van-Tintelen G, Van-Zutphen LFM, Beynen AC. Influence of amount of dietary fat and protein on esterase-1 (ES-1) activities of plasma and small intestine in rats. Br J Nutr 1992; 67: 379-390.
26. Alexon SE, Finlay TH, Hellman U, Svensson LT, Diczfalusy U, EggertseG. Molecular cloning and identification of a rat serum carboxylesterase expressed in the liver. J Biol Chem 1994 ;269 :17118-17124.
27. Ellinghaus P , Seedorf U, Assmann G. Cloning and sequencing of a novel murine liver carboxylesterase cDNA. Biochim Biophys Acta 1998; 1397:175-179.
28. Harrisson EH. Lipases and carboxylesterases: possible roles in the hepatic metabolism of retinol. Ann Rev Nutr 1998 ;18 :259-276.
29. Diczfalusy MA , Bjorkhem I, Einarsson K, Alexon SEH. Formation of fatty acid ethyl esters in rat liver microsomes. Evidence for a key role for acyl-CoA : ethanol O-acyltransferase .Eur J Biochem 1999; 259:404-411.
30. Sanghani SP, Davis WI , Dumaual NG, Mahrenholz A, Bosron WF Identification of microsomal rat liver carboxylesterases and their activity with retinyl palmitate. Eur J Biochem 2002;269:4387-4398
31. Johnsen H, Odden E, Lie O, Johnsen BA , Fonnum NG .Metabolism of T-2 toxin by rat liver carboxylesterase. Biochem Pharmacol 1986;35:1469- 1473
32. Tang J , Chambers JE. Detoxication of paraoxon by rat liver homogenate and serum carboxylesterases and esterases. J Biochem Mol Toxicol 1999;13:261-268
33. Kudo S, Umehara K ,Hosokawa M, Miyamoto G ,Chiba K, Satoh T. Phenacetin deacetylase activity in human liver microsomes:distribution, kinetics ,and chemical inhibition and stimulation .J Pharmacol Exper Ther 2000;294:80-87
34. Alexon SEH, Diczfalusy M , Halldin M, Swedmark S. Involvement of liver carboxylesterases in the in vitro metabolism of lidocaine. Drug Metab Dispos 2002;30:643-647
35. Xie M ,Yang D,Wu M ,Xue B, Yan B. Mouse liver and kidney carboxylesterase (M-LK) rapidly hydrolyzes antitumour prodrug irinotecan and the N-terminal three quarter sequence determines substrate specificity. Drug Metab Dispos 2003;31:21-27.
36. Pease DC. Histological Techniques for Electron Microscopy. 2nd ed., Academic Press, N.Y. & London, 1964: 14-81.
37. Nachlas M.M., Tsou, K.C., De Souza, E., Cheng, C.S., Seligman A.M. (1957). Cytochemical demonstration of succinic dehydrogenase by the use of a new p-nitrophenyl substituted ditetrazole. J. Histochem. Cytochem. 1957; 5: 420-436.
38. Meijer AEFH. Improved histochemical method for the demonstration of the activity of α-glucan phosphorylase.I. The use of glycosyl acceptor dextran. Histochemie 1968; 12: 244-252.
39. Pearse AGE. Histochemistry Theoretical and Applied. 3rd ed. Churchill Livingstone Edinburgh & London 1972; 2: 761-807.
40. Al-Khalisi MH, AL-Khafaj FA, Al-Azzawi HT. The distribution of non- specific esterases in the laminae of the grey matter of the rat’ s spinal cord under normal circumstances and during pain.J Fac Med Baghdad 1999;41:528-532
41. Satoh T, Hosokawa M. The mammalian carboxylesterases:from molecules to functions. Annu Rev Pharmacol Toxicol 1998;38:257-188
How to Cite
AL-DULAYMI, Shehab H. Mehenna; AZZAWI, Hani T.. THE METABOLIC ACTIVITY OF THE ADULT RABBIT CHOROID PLEXUS: AN ENZYMATIC HISTOCHEMICAL APPROACH. Gezira Journal of Health Sciences, [S.l.], v. 1, n. 2, jan. 2005. ISSN 1810-5386. Available at: <>. Date accessed: 18 sep. 2019.