Determination of Total Phenolic Content and Antioxidant Activity of Roselle (Hibiscus sabdariffa L.) Calyx and Epicalyx Ethanolic (Alcoholic) Extract

  • Nizar Sirag Department of Pharmacognosy, Faculty of Pharmacy, University of Gezira, Sudan.
  • Elhadi MM Department of Pharmacognosy, Faculty of Pharmacy, University of Gezira, Sudan
  • Algaili M Algaili Department of Pathology, Faculty of Medicine, University of Gezira, Sudan
  • Mohamed Ohaj Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, University of Gezira, Sudan

Abstract

Background: Medicinal plants contain physiologically active ingredients that over the years have been exploited in traditional medicine for the treatment of various ailments.


 Objectives: This study was undertaken to investigate the total phenolic content and antioxidant capacity of Roselle (Hibiscus sabdariffa) calyx and epicalyx ethanolic extract.


 Methods: The total phenolic content was estimated by Folin Ciocalteau method using gallic acid as standard while the antioxidant capacity was determined based on the plant extract to scavenge DPPH radical.


Results: The total phenolic content was found to be 41.07 mg gallic acid equivalent /g .The extract exhibited a significant measurable dose dependent inhibition of the DPPH activity. At a concentration of 250µg/ml, sabdariffa calyx epicalyx ethanolic extract scavenged 86% of DPPH radical whereas 125 and 50 µg/ml caused 53% and 23% DPPH inhibition respectively and a very mild inhibition was produced at a concentration of 5 µg/ml. 


Conclusion: It can be concluded that sabdariffa calyx epicalyx ethanolic could be a potential source of antioxidant principles.

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Published
2014-06-01
How to Cite
SIRAG, Nizar et al. Determination of Total Phenolic Content and Antioxidant Activity of Roselle (Hibiscus sabdariffa L.) Calyx and Epicalyx Ethanolic (Alcoholic) Extract. Gezira Journal of Health Sciences, [S.l.], v. 10, n. 1, june 2014. ISSN 1810-5386. Available at: <http://journals.uofg.edu.sd/index.php/gjhs/article/view/337>. Date accessed: 28 nov. 2020.
Section
Articles