Determination of Gossypol Content in Cottonseed Oil and Cake by HPLC and GC/ms

  • Ayia M. EL-Hassan Gezira University, Sudan
  • Salah M. Nour Agricultural Research Corporation (ARC) Wad madani, Sudan
  • mohammed O.Babiker (3) Department of Applied Chemistry and Chemical Technology, Faculty of Engineering and Technology, University of Gezira, Sudan


     This study investigated gossypol content in cottonseed oil and cottonseed cake of Bt cottonseed (Seeni1) and non-Bt cottonseed (Barakat 90, Abdeen, Mixed) in oil and cake by difference methods. Among the all non-Bt cottonseed varieties Barakat (90) had shown higher amount of gossypol content in oil and cake (470 and 467) ppm (890 and 897) ppm by using HPLC and GC/MS respectively. Moreover, Bt cottonseed variety (Seeni1) had shown lower amount of gossypol content using both methods (320 and 326) ppm and (640 and 635) ppm by using HPLC and GC/MS respectively. From the present study, we observed that was no significant difference in gossypol content between Bt cottonseed and non-Bt cottonseed varieties. Bt cottonseed variety has lower amount of gossypol being of more economical importance to farmers having cotton as their cash crop.


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How to Cite
EL-HASSAN, Ayia M.; M. NOUR, Salah; O.BABIKER, mohammed. Determination of Gossypol Content in Cottonseed Oil and Cake by HPLC and GC/ms. Gezira Journal of Engineering and Applied Sciences, [S.l.], v. 15, n. 1, p. 79-90, june 2021. ISSN 1858-5698. Available at: <>. Date accessed: 27 oct. 2021.