Production and characterization of Pectic enzymes from three fungi

  • Awad M. Abdel-Rahim Department of chemistry and Biology, Fac. of Educ. Unv. of Gezira
  • Amina A. Elmustafa 1Center of Biosci. And Biotech. Fac. of Eng. & technology


Pectins are compounds  that  are resistant   to  enzymatic  cleavage  in

most  organisms  and  plants, Studies  were  therefore  made towards finding

methods for hydrolyzing these compounds. One such approach  is enzymatic

hydrolysis.  Three fungi   (Trichoderma   viride,    Asperigillus     niger   and

penicillum   digitatum)  are   used   for  production   of   pectinase  enzymes,

Two  methods (reducing  sugar and viscometary)  were  used  for  measuring

enzymes activities. Pectin and sodium polypectate compounds were used  as

substrates. The  production of  pectinases by the three fungi was investigated

in culture media. T. viride and P. digitatum  gave  maximum   production  of

pectinase  enzymes  after  two  week  and  A. niger  gave  it  after  one week.

Purification   by   ammonium    sulphate   precipitation,     showed   that   the

maximum  pectinase  activity  was  at   80%   concentration for both fungi A

niger and P. digitatum. By using  gel  electrophoresis, five bands were found

to   give   pectinase  enzymes   activities. The   present   study   showed    the

importance   of   fungi   as   sources  of  enzymes and recommends that more

studies  must   be   done   in   the  field of  biotechnology to produce glucose

 from natural products by  using fungi.



Abdel- Rahim, A. M. (1980), Studies on Sesame Leaf Spot
caused by Xanthomonas sesame (Sabet and Dowson), Ph.D.
thesis, Department of Botany and Plant Technology, Imperial
Collage of Science and Technology. London.
Almustafa, A. A. and Abdel- Rahim, A. M. (2013). Production
and characterization of cellulolytic enzymes from three fungi.
Gez. J. of Eng. and Appl Sci. 10 (1): 1 — 19.

Ayers, W. Papavizas, GA C, and Diem, A. F. (1966),
Polygalacturonate Trans - Eleminase and Polygalacturonase
Production b Rhizoctonia solani Journal of Phytopathology. 56:
925 930.
Bateman, D and Miller, R. L. (1966}, Pectic Enzymes in Tissue
Degradation. Annual Review of Plant Pathology, 4: 119 124.
Birch, G.G, Blakebrough, N. and Parker, J. (1981). Enzyme and
Food Processing. Applied science publisher LTD„ England. Pp 5-
Braverman, J. B. F. (1974) Introduction to Biochemistry of
Food. 3rd. ed, , Elsevier publishing Company. London. Pp 31.
Castaldo, D.; qualiquuolo, L; Servillo, L; Balestrieri, C. and
Giouane, A. (1989). Isolation and Characterization of
Pectinmethylesterase from Apple Fruit. Journal of Food Science,
54(3): 653 – 673.
coral, G.; B. M. Nisa, O. and Hatice, G (2001). Some
properties of Crude Carboxymethyl Cellulase of Asperigillus
niger. Turkish Journol of Biology, 26: 209 213,
Danial, J. and Emmanuel, E. (2011) , Effect of Carbon and
Nitrogen sources on Polygalacturanase Production by viride
Isolate Tar Sand in Ondo State (Nigeria). 7 (3) : 153 – 158.
Desouky, E. M, (2007), Production of cellulase by Penicillum
hordei and by Asperigillus ustus under solid state fermentation
condition, Egyptian Journal of Microbiology, 17 : 6 - 64.
Forester, H. (1988). Pectinase from Phytophthora infestants.
Journal of Enzymology. 161:307-355.

Gupta, V. K. and Ayyachamy, M. (2012), Biotechnology of
Fungal Genesc Science publisher, England. Pp 53-71.
Joshi, V. K. Mukesh, P. and Neerja, R. (2011) Purification and
Characterization of Pectinase produced from Apple Pomace and
Evaluation of its Efficacy in Fruit Juice Extraction and
Clarification. Indian Journal of Natural Products and Resources,
2(2): 189-197.
Kabli, S. A. (2007). Purification and Characterization of
Protopectinase produced by Kluyveromyces marxianus, JKAU:
Sci, vol. 19: 139 - 153.
Kareem, O. S. and Adebowalej A. A. (2002). Clarification of
Orange Juice by Crude Fungi Pectinase from Citrus Peel.
Nigerian Food Journal. 25 (1): 130 – 137.
How to Cite
M. ABDEL-RAHIM, Awad; A. ELMUSTAFA, Amina. Production and characterization of Pectic enzymes from three fungi. Gezira Journal of Engineering and Applied Sciences, [S.l.], v. 8, n. 2, p. 18 - 35, nov. 2013. ISSN 1858-5698. Available at: <>. Date accessed: 29 nov. 2022.