Production and characterization of Pectic enzymes from three fungi
Pectins are compounds that are resistant to enzymatic cleavage in
most organisms and plants, Studies were therefore made towards finding
methods for hydrolyzing these compounds. One such approach is enzymatic
hydrolysis. Three fungi (Trichoderma viride, Asperigillus niger and
penicillum digitatum) are used for production of pectinase enzymes,
Two methods (reducing sugar and viscometary) were used for measuring
enzymes activities. Pectin and sodium polypectate compounds were used as
substrates. The production of pectinases by the three fungi was investigated
in culture media. T. viride and P. digitatum gave maximum production of
pectinase enzymes after two week and A. niger gave it after one week.
Purification by ammonium sulphate precipitation, showed that the
maximum pectinase activity was at 80% concentration for both fungi A
niger and P. digitatum. By using gel electrophoresis, five bands were found
to give pectinase enzymes activities. The present study showed the
importance of fungi as sources of enzymes and recommends that more
studies must be done in the field of biotechnology to produce glucose
from natural products by using fungi.
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